Difference between revisions of "Part:BBa K1739003:Design"

 
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<partinfo>BBa_K1739003 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Sequence optimisation
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This BioBrick has been optimised for use in the VS45 strain of E.coli containing deletions that prevent amyloid from binding to the outside of the cell and increase the rate of protein exiting the cell via the curli export system.
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===Source===
 
===Source===
  
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It comes from a genomic sequence of Saccharomyces cerevisiae
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It comes from a genomic sequence of Saccharomyces cerevisiae (bakers yeast).
  
 
===References===
 
===References===

Latest revision as of 13:44, 16 September 2015

Sequence coding for Envirowire: Sup35NM with N-terminal CsgAss and C-terminal cytb562


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 718


Design Notes

This BioBrick has been optimised for use in the VS45 strain of E.coli containing deletions that prevent amyloid from binding to the outside of the cell and increase the rate of protein exiting the cell via the curli export system.

Source

It comes from a genomic sequence of Saccharomyces cerevisiae (bakers yeast).

References