Difference between revisions of "Part:BBa K1587004:Design"
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===Design Notes=== | ===Design Notes=== | ||
p(Bla) is followed by NheI restriction site and ccr is followed by XhoI restriction site | p(Bla) is followed by NheI restriction site and ccr is followed by XhoI restriction site | ||
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| − | |||
===Source=== | ===Source=== | ||
| − | + | Sequences are from ''Clostridium acetobutylicum'' : <br> | |
| − | - hbd http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2831733&to=2832581&strand=true | + | - hbd http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2831733&to=2832581&strand=true <br> |
- crt http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2835666&to=2836451&strand=true | - crt http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2835666&to=2836451&strand=true | ||
| − | + | Sequences are from ''E. coli'' <br> | |
| − | - atoB http://www.ncbi.nlm.nih.gov/nuccore/NC_020990.1?report=fasta&from=6738960&to=6740186&strand=true | + | - atoB http://www.ncbi.nlm.nih.gov/nuccore/NC_020990.1?report=fasta&from=6738960&to=6740186&strand=true <br> |
- tesB http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=474301&to=475161&strand=true | - tesB http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=474301&to=475161&strand=true | ||
| − | Sequence is from Streptomyces caulinus : | + | Sequence is from ''Streptomyces caulinus'' :<br> |
- ccr | - ccr | ||
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===References=== | ===References=== | ||
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| + | - El-Hussiny A, J. Bacteriol, 2013. Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli.<br> | ||
| + | - Mukesh S, J Agric Food Chem, 2014. Metabolic Engineering of Escherichia coli for Production of Butyric Acid<br> | ||
| + | - Atsumi S, 2008, Metab Eng. Metabolic engineering of Escherichia coli for 1-butanol production. Metabolic Engineering 10:305–311. | ||
Latest revision as of 15:01, 17 September 2015
Butyrate synthesis pathway regulated by constitutive promoter p(Bla)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 36
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1717
Illegal BamHI site found at 3634
Illegal XhoI site found at 1407 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 332
Illegal AgeI site found at 1254
Illegal AgeI site found at 2900 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1752
Illegal BsaI.rc site found at 2169
Illegal BsaI.rc site found at 3237
Illegal SapI site found at 123
Illegal SapI.rc site found at 3249
Design Notes
p(Bla) is followed by NheI restriction site and ccr is followed by XhoI restriction site
Source
Sequences are from Clostridium acetobutylicum :
- hbd http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2831733&to=2832581&strand=true
- crt http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2835666&to=2836451&strand=true
Sequences are from E. coli
- atoB http://www.ncbi.nlm.nih.gov/nuccore/NC_020990.1?report=fasta&from=6738960&to=6740186&strand=true
- tesB http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=474301&to=475161&strand=true
Sequence is from Streptomyces caulinus :
- ccr
The strong RBS and Terminator were used according to respectively BBa_B0030 and BBa_B1006
References
- El-Hussiny A, J. Bacteriol, 2013. Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli.
- Mukesh S, J Agric Food Chem, 2014. Metabolic Engineering of Escherichia coli for Production of Butyric Acid
- Atsumi S, 2008, Metab Eng. Metabolic engineering of Escherichia coli for 1-butanol production. Metabolic Engineering 10:305–311.