Difference between revisions of "Part:BBa K1688002"

Latest revision as of 22:25, 18 September 2015

RhlA Gene

The enzymes responsible for producing mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The rhlA gene coding for the enzyme RhlA creates the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomonas aeruginosa. The biobrick Ba_K1688002 is an assembly of RBS with BBa_K1331001.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 87
Illegal BamHI site found at 647
Illegal XhoI site found at 823
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 312
Illegal BsaI.rc site found at 496

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 __NOTOC__
 <partinfo>BBa_K1688002 short</partinfo>
-The enzymes responsible for producing the mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The mono- rhamnolipids consist of a hydrophobic tail and a hydrophilic head. The RhlA gene codes for the fatty acid tail and it has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick from team Nankai BBa_K1331001 consists of gene RhlA whereas the biobrick BBa_K1688002  consist of a ribosomal binding site and a gene coding  RhlA.
+The enzymes responsible for producing mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The <i>rhlA</i> gene coding for the enzyme RhlA creates the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from <i>''Pseudomonas aeruginosa''</i>. The  biobrick [https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]  is an assembly of RBS with [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001].
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