Difference between revisions of "Part:BBa K1696016"
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− | + | Mf-ssrA tag2 is one of the identified variants from mf-ssrA tag1 (BBa_K1696013) that could maintain steady-state RFP levels and display a range of mf-Lon-dependent degradation rates. Among all other tags, the proteolysis ability of this tag is a little bit weaker than mf-ssrA tag1 (BBa_K1696013) while stronger than the other variants. | |
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+ | == Reference: == | ||
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+ | Cameron D E, Collins J J. Tunable protein degradation in bacteria.[J]. Nature Biotechnology, 2014, 32(12). | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 14:34, 17 September 2015
Mf-ssrA Tag2
Mf-ssrA tag2 is one of the identified variants from mf-ssrA tag1 (BBa_K1696013) that could maintain steady-state RFP levels and display a range of mf-Lon-dependent degradation rates. Among all other tags, the proteolysis ability of this tag is a little bit weaker than mf-ssrA tag1 (BBa_K1696013) while stronger than the other variants.
Reference:
Cameron D E, Collins J J. Tunable protein degradation in bacteria.[J]. Nature Biotechnology, 2014, 32(12).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]