Difference between revisions of "Part:BBa K1631020:Experience"
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===Applications of BBa_K1631020=== | ===Applications of BBa_K1631020=== | ||
| + | <p>We constructed new composite CheZ generators(CheZ strong1 generator [[Part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator[[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ | ||
| + | expression. </p> | ||
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| + | [[File:Nakamura coef of diffusion.png|600px|thumb|center|alt text]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 00:12, 19 September 2015
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Applications of BBa_K1631020
We constructed new composite CheZ generators(CheZ strong1 generator Part:BBa_K1631018, CheZ strong2 generatorpart:BBa_K1631019, Medium CheZ generatorpart:BBa_K1631020) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of E.coli quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ expression.
User Reviews
UNIQe73d66f1512442ad-partinfo-00000000-QINU UNIQe73d66f1512442ad-partinfo-00000001-QINU