Difference between revisions of "Part:BBa K1729001"

 
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
−
__NOTOC__
 
 
<partinfo>BBa_K1729001 short</partinfo>
 
<partinfo>BBa_K1729001 short</partinfo>
  
−
The translational unit includes the Kozak and full optimized coding sequence for the Saccharomyces cerevisiae MRPS12 gene, which encodes a ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal, which is presumably removed in the mitochondrion. The resultant protein is a uS12 homologue.
+
This translational unit includes the Kozak and codon-optimized sequence for the ''Saccharomyces cerevisiae'' [http://www.yeastgenome.org/locus/S000005319/overview ''MRPS12''] gene, which encodes a ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal (MLS), which is presumably removed in the mitochondrion. The resultant protein is a homologue of the highly conserved uS12 ribosomal protein (Ban ''et al''.), which is essential for ribosome function.
 +
 
 +
 
 +
The part sequence corresponds to nucleotides 694840-694361 on chromosome XIV of the ''S. cerevisiae'' S288C reference genome. See also: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1729002 BBa_K1729002]
 +
 
 +
 
 +
[[File:MRPS12 Seq.jpg|900 px|]]
 +
<div class="center" style="width: auto; margin-left: auto; margin-right: auto;">Screen capture from SnapGene&reg;</div>
 +
 
 +
 
 +
'''References'''
 +
 
 +
Ban, Nenad ''et al''. “A New System for Naming Ribosomal Proteins.” Cur Opin Struct Biol 24 (2014): 165–169.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:56, 19 September 2015

Yeast mitochondrial ribosomal protein MRPS12

This translational unit includes the Kozak and codon-optimized sequence for the Saccharomyces cerevisiae [http://www.yeastgenome.org/locus/S000005319/overview MRPS12] gene, which encodes a ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal (MLS), which is presumably removed in the mitochondrion. The resultant protein is a homologue of the highly conserved uS12 ribosomal protein (Ban et al.), which is essential for ribosome function.


The part sequence corresponds to nucleotides 694840-694361 on chromosome XIV of the S. cerevisiae S288C reference genome. See also: BBa_K1729002


MRPS12 Seq.jpg

Screen capture from SnapGene®


References

Ban, Nenad et al. “A New System for Naming Ribosomal Proteins.” Cur Opin Struct Biol 24 (2014): 165–169.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]