Difference between revisions of "Part:BBa K1597004"

Latest revision as of 14:38, 18 September 2015

slrR with strong promoter and RBS

SlrR is an important transcriptional regulator in Bacillus subtilis. SlrR represses the sigma(D)-dependent flagellar genes and activates the eps and yqxM operons. Therefore it is has a controlling factor in biofilm formation. By overexpressing slrR, with the use of a strong promoter (BBa_K090504) and a strong RBS (BBa_K780001)(BBa_K780001), a robust biofilm can be formed. slrR with the promter and RBS was integrated via BBa_K823023 and via BBa_K1597001.

slrR mutant with an overexpression of slrR shows a bigger biofilm than the wildtype B. subtilis 3610 ΔcomI control. This phenotype could be due to the overexpression of the eps operon, leading to many extracellular polysaccharide substances. Resulting in a larger biofilm. The combination of the abrB knockout and slrR+ resulted in a biofilm size to the control. The process behind the this interaction is not known, however comparing these phenotypes clearly shows an interaction.

alt text

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1597004 short</partinfo>
-SlrR is an important transcriptional regulator in Bacillus subtilis. SlrR represses the sigma(D)-dependent flagellar genes and activates the eps and yqxM operons. Therefore it is has a controlling factor in biofilm formation. By overexpressing SlrR, with the use of a strong promoter (BBa_K090504) and a strong RBS (BBa_K780001), a robust biofilm can be formed.
+SlrR is an important transcriptional regulator in Bacillus subtilis. SlrR represses the sigma(D)-dependent flagellar genes and activates the eps and yqxM operons. Therefore it is has a controlling factor in biofilm formation. By overexpressing ''slrR'', with the use of a strong promoter <html><a href="https://parts.igem.org/Part:BBa_K090504"> (BBa_K090504)</a></html> and a strong RBS <html><a href="https://parts.igem.org/Part:BBa_K780001"> (BBa_K780001)</a></html>(BBa_K780001), a robust biofilm can be formed. ''slrR'' with the promter and RBS was integrated via <html><a href="https://parts.igem.org/Part:BBa_K823023"> BBa_K823023</a></html> and via <html><a href="https://parts.igem.org/Part:BBa_K1597001"> BBa_K1597001</a></html>.
+''slrR'' mutant with an overexpression of ''slrR'' shows a bigger biofilm than the wildtype ''B. subtilis 3610 ΔcomI'' control. This phenotype could be due to the overexpression of the eps operon, leading to many extracellular polysaccharide substances. Resulting in a larger biofilm.
+The combination of the abrB knockout and ''slrR''+ resulted in a biofilm size to the control. The process behind the this interaction is not known, however comparing these phenotypes clearly shows an interaction.
+[[File:Slrr.jpg|200px|thumb|left|alt text]]
 <!-- Add more about the biology of this part here