Difference between revisions of "Part:BBa K1789008:Design"

Latest revision as of 02:00, 19 September 2015

TALE1

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2082
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1075

Design Notes

This part is designed by ourselves based on the sequence of BM1 in our project.

NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard. We then finished the standardization of this part b ourselves.

Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids

The protocol can be reached in our wiki's protocol page.

Source

This part is built by NJU_CHINA using Golden Gate technique. We standardized this part by ourselves.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1789008 short</partinfo>
@@ Line 7: / Line 6: @@
 ===Design Notes===
-The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
+This part is designed by ourselves based on the sequence of BM1 in our project.
+NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard. We then finished the standardization of this part b ourselves.
+[[File:TALE1.jpg|800px|]]
+Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids
+The protocol can be reached in our wiki's protocol page.
 ===Source===
-This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves.
+This part is built by NJU_CHINA using Golden Gate technique. We standardized this part by ourselves.
 ===References===