Difference between revisions of "Part:BBa K1859001:Experience"
 
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===Applications of BBa_K1859001===
 
===Applications of BBa_K1859001===
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<html>
  
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<p>
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<a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015]
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</a> .
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</p>
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<p>
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We attached this part[BBa_1859001] to 5 'sides of
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His-RFP without stop codon
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" > [BBa_K1332002]
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</a> .
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Then, 
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it was connected to each 5 'sides of
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004]
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</a>
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and
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006]
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</a>.
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In addition,Lacl
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
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, RBS
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0034" >[BBa_B0034] </a>
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and DT
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a>
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was conbined with them, making
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859027" >[BBa_K1859027] </a>
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and
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" >[BBa_K1859025] </a>
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.
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859027" >[BBa_K1859027] </a>
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is the generator that express long-chain protein with keeping functionality of the protein.
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" >[BBa_K1859025] </a>
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is the generator to improve circular efficiency of mRNA including His-RFP.
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</p>
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</html>
 
===User Reviews===
 
===User Reviews===
 
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<html>
 
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<p>
 
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You attach this part to 5 'sides of any parts.
 
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In addition, you couple mRNA circularization device (3'side) in 3 'sides.
 
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When this device is expressed in E.coli, Curcular mRNA including the linker is made.
 
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The efficiency that mRNA is circularized changes by sequence of the mRNA.
 
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We let mRNA of RFP circularize and produce a long-chain protein, but the functionality was lost.
 
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It is a problem to make a long-chain protein with keeping functionality.
 
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</p>
 
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</html>
 

Latest revision as of 04:54, 25 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859001

[iGEM gifu 2015] .

We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, it was connected to each 5 'sides of [BBa_K1859004] and [BBa_K1859006] . In addition,Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] was conbined with them, making [BBa_K1859027] and [BBa_K1859025] . [BBa_K1859027] is the generator that express long-chain protein with keeping functionality of the protein. [BBa_K1859025] is the generator to improve circular efficiency of mRNA including His-RFP.

User Reviews

UNIQ35f8fc99f2ebb870-partinfo-00000001-QINU UNIQ35f8fc99f2ebb870-partinfo-00000002-QINU