Difference between revisions of "Part:BBa K1659211:Design"
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===References=== | ===References=== | ||
[1] SnapGene software (from GSL Biotech; available at snapgene.com) | [1] SnapGene software (from GSL Biotech; available at snapgene.com) |
Latest revision as of 10:21, 14 September 2015
Dispersin B fused with DsbA, with mutation at nucleotide 636 (T to C)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 316
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 472
Design Notes and Sources
This part is a modified version of BBa_K1659201, where thymine 636 was mutated into a cytosine, conserving the His-212 which it encodes for. The site-directed mutagenesis was performed using a modified NEB Q5 Mutagenesis Protocol, which is fully described in our team's [http://2015.igem.org/Team:Oxford/Protocols Protocols Page].
References
[1] SnapGene software (from GSL Biotech; available at snapgene.com)