Difference between revisions of "Part:BBa K1713000:Design"

 
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<partinfo>BBa_K1713000 short</partinfo>
 
<partinfo>BBa_K1713000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
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Compared with BBa_K094120, We have 1 site mutation and 1 gap which lead to two more restriction enzyme cutting sites, HindIII and BamHI.
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===Source===
 
===Source===
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It was reconstructed through PCR with BBa_K094120 served as template and a pair of primers with two mutation.
  
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===References===
 
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.
 
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.
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===References===
 

Latest revision as of 16:07, 18 September 2015

Plac/ara-1, a hybrid promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compared with BBa_K094120, We have 1 site mutation and 1 gap which lead to two more restriction enzyme cutting sites, HindIII and BamHI.

Source

It was reconstructed through PCR with BBa_K094120 served as template and a pair of primers with two mutation.

References

Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.