Difference between revisions of "Part:BBa K1602009"
 
(17 intermediate revisions by 2 users not shown)
Line 9: Line 9:
 
     </head>
 
     </head>
 
     <body>
 
     <body>
−
         <h1>D-xylose dehydrogenase xylB</h1>
+
         <h1><small>D</small>-Xylose dehydrogenase - xylB</h1>
 
         <table>
 
         <table>
 
             <colgroup width=50% span="2">
 
             <colgroup width=50% span="2">
 
             <tr>
 
             <tr>
 
                 <td>
 
                 <td>
−
                 <b>xylB</b> xylB is a  
+
                 <i>xylB</i> encodes for a NAD<sup>+</sup> <small>D</small>-xylose dehydrogenase from <i>Caulobacter crescentus</i>. It
−
                </td>
+
                 catalyzes the reaction of <small>D</small>-xylose to <small>D</small>-xylonolactone. The sequence
−
                 <td>
+
                is codon optimized for the usage in <i>E. coli</i>.
−
                    <img style="width: 300px; height: 300px; align: center; margin-left: 100px; margin-right:100px" alt="" src="img/citrate_synthase_gltA.png">      
+
 
                 </td>
 
                 </td>
 +
               
 
             </tr>
 
             </tr>
 
             <tr>
 
             <tr>
Line 25: Line 25:
 
                 </td>
 
                 </td>
 
                 <td>
 
                 <td>
−
                    <b>Figure 1</b> cis-aconitate decarboxylase (coded by cadA)
+
                <!-- <img style="width: 231px; height: 900px; margin-left: 15px; margin-right: 15px;" alt="" src=" https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png">   -->
 +
<img class="shrinkToFit transparent" alt="https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png" src="https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png" height="384" width="231">
 
                 </td>
 
                 </td>
 
             </tr>
 
             </tr>
Line 92: Line 93:
  
 
===<h2>Sequence and Features</h2>===
 
===<h2>Sequence and Features</h2>===
−
<partinfo>BBa_K1602001 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K1602009 SequenceAndFeatures</partinfo>

Latest revision as of 21:29, 18 September 2015

D-Xylose dehydrogenase - xylB

xylB encodes for a NAD+ D-xylose dehydrogenase from Caulobacter crescentus. It catalyzes the reaction of D-xylose to D-xylonolactone. The sequence is codon optimized for the usage in E. coli.
https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png

Characteristics


Molecular Weight 26641.48
Residues 248
Charge -4.0
Isoelectric Point 5.1521
A280 Molar Extinction Coefficients 36440 (reduced) 36690 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]