Difference between revisions of "Part:BBa K1682011"

(→‎NsrR - Nitric oxide sensitive PyeaR repressor protein)
 
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=NsrR - Nitric oxide sensitive P<sub>yeaR</sub> repressor protein=
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NsrR - Nitric oxide sensitive <i>P<sub>yeaR</sub></i> repressor protein
  
 
===Biology of NsrR===
 
===Biology of NsrR===
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[[File:HKUST-Rice 2015 pyeaRMechanismm.PNG|thumb|500px|center|<b>Fig.1 </b>Nitrate sensing mechanism of P<sub>yeaR</sub>]]
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[[File:Team HKUST-Rice 2015 Nitrate PyeaR trans.gif|thumb|500px|center|<b>Fig.1 </b>Nitrate sensing mechanism of P<sub>yeaR</sub>]]
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NsrR protein is a nitrate sensitive transcriptional repressor.
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When there is nitrate, it will be oxidized to nitric oxide by nitrate oxidase, which in turns relieve the repression of NsrR on PyeaR promoter.
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<i>Escherichia coli</i> (<i>E. coli</i>) detects environmental nitrate by the <i>yeaR-yoaG</i> operon. According to Figure 1, <i>P<sub>yeaR</sub></i> (Lin, <i>et al.</i>, 2007) is regulated by the Nar two-component regulatory system (Nohno <i>et al.</i>, 1989; Li <i>et al.</i>, 1987) and NsrR nitric oxide sensitive repressor protein (Partridge <i>et al.</i>, 2009). When there is nitrate or nitrite, the repression from the Nar system on <i>P<sub>yeaR</sub></i> will be relieved due to the binding between the two. On the other hand, some nitrate will be converted into nitric oxide by nitrate reductase. Nitric oxide will bind to the NsrR protein and relieve the repression on <i>P<sub>yeaR</sub></i>. As a result, any genes that are downstream of <i>P<sub>yeaR</sub></i> will be expressed.
  
  
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 03:42, 19 September 2015


NsrR

NsrR - Nitric oxide sensitive PyeaR repressor protein

Biology of NsrR

Fig.1 Nitrate sensing mechanism of PyeaR

Escherichia coli (E. coli) detects environmental nitrate by the yeaR-yoaG operon. According to Figure 1, PyeaR (Lin, et al., 2007) is regulated by the Nar two-component regulatory system (Nohno et al., 1989; Li et al., 1987) and NsrR nitric oxide sensitive repressor protein (Partridge et al., 2009). When there is nitrate or nitrite, the repression from the Nar system on PyeaR will be relieved due to the binding between the two. On the other hand, some nitrate will be converted into nitric oxide by nitrate reductase. Nitric oxide will bind to the NsrR protein and relieve the repression on PyeaR. As a result, any genes that are downstream of PyeaR will be expressed.




Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 154
  • 1000
    COMPATIBLE WITH RFC[1000]