Difference between revisions of "Part:BBa K1628003"

Latest revision as of 09:04, 18 September 2015

BJ27UP

Promoter BJ27UP is an artificially synthesized promoter. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in B. amyloliquefaciens NK-1 (showed in Figure 1), the strength of BJ27UP is stronger than Pbca but is still too weak compared with other promoters.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1628003 short</partinfo>
-Promoter BJ27UP is an artificially synthesized promoter. According to our promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), the strength of BJ27UP is stronger than Pbca but is still too weak compared with other promoters.
+Promoter BJ27UP is an artificially synthesized promoter. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), the strength of BJ27UP is stronger than Pbca but is still too weak compared with other promoters.
-[[File:promoter_NK.png]]
+[[File:promoter4_NK.png]]
 <!-- Add more about the biology of this part here