Difference between revisions of "Part:BBa K1628006"
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| − | Promoter P43 is a strong promoter in Bacillus subtilis 168. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in B. amyloliquefaciens NK-1. | + | Promoter P43 is a strong promoter in ''Bacillus subtilis'' 168. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in ''B. amyloliquefaciens'' NK-1. |
| − | [[File: | + | [[File:promoter4_NK.png]] |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:07, 18 September 2015
P43
Promoter P43 is a strong promoter in Bacillus subtilis 168. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in B. amyloliquefaciens NK-1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]