Difference between revisions of "Part:BBa K1789006:Design"

 
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===Design Notes===
 
===Design Notes===
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This part is designed to contain two basic short sequences which can bind TALE1 and TALE2(3) specifically. These two basic sequences are repeated with no space by ten times, so it can bind more TALE protein and the spatial location of each pair of TALE1 and TALE2(3) is almost opposite.
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The length of DNA BMs and intervening sequences is designed based on the typical ''in vivo'' B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.
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This scaffold is designed to avoid steric hindrance and to ensure the spatial orientation of enzymes. A 6-bp intervening sequence was added between BMs.
  
 
===Source===
 
===Source===

Latest revision as of 02:21, 19 September 2015

SCAF2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 8
    Illegal AgeI site found at 50
    Illegal AgeI site found at 92
    Illegal AgeI site found at 134
    Illegal AgeI site found at 176
    Illegal AgeI site found at 218
    Illegal AgeI site found at 260
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length of DNA BMs and intervening sequences is designed based on the typical in vivo B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.

This scaffold is designed to avoid steric hindrance and to ensure the spatial orientation of enzymes. A 6-bp intervening sequence was added between BMs.

Source

This part is synthetized by viewsolid biotech firm (Beijing, China).

References