Difference between revisions of "Part:BBa K1628101"
Songxinhao (Talk | contribs) |
Songxinhao (Talk | contribs) |
||
| Line 2: | Line 2: | ||
<partinfo>BBa_K1628101 short</partinfo> | <partinfo>BBa_K1628101 short</partinfo> | ||
| − | ''pgsB'' is a gene responsible for γ-PGA synthesis in ''pgsBCA'' operon. Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure | + | ''pgsB'' is a gene responsible for γ-PGA synthesis in ''pgsBCA'' operon. Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 1). |
| − | [[File: | + | [[File:pgsBCA1_NK.png]] |
| + | |||
| + | Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type ''Bacillus amyloliquefaciens'' NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in ''E. coli''. We constructed different expression vectors containing ''pgsBCA'' genes and transformed them separately into ''E.coli'' JM109. The average production of γ-PGA in ''E. coli'' JM109 is around 0.5g/L. | ||
| + | |||
| + | [[File:pgsBCA2_NK.png]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 08:03, 18 September 2015
pgsB
pgsB is a gene responsible for γ-PGA synthesis in pgsBCA operon. Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 1).
Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type Bacillus amyloliquefaciens NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in E. coli. We constructed different expression vectors containing pgsBCA genes and transformed them separately into E.coli JM109. The average production of γ-PGA in E. coli JM109 is around 0.5g/L.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]