Difference between revisions of "Part:BBa K1628001"
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<partinfo>BBa_K1628001 short</partinfo> | <partinfo>BBa_K1628001 short</partinfo> | ||
| − | Promoter Pbca is the original promoter of pgsBCA operon. According to | + | Promoter Pbca is the original promoter of ''pgsBCA'' operon. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak. |
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| + | [[File:promoter4_NK.png]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 08:56, 18 September 2015
Pbca
Promoter Pbca is the original promoter of pgsBCA operon. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]