Difference between revisions of "Part:BBa K1595022:Design"
(→Design Notes) |
|||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1595022 short</partinfo> | <partinfo>BBa_K1595022 short</partinfo> | ||
Line 10: | Line 9: | ||
Has internal PstI cutsite. | Has internal PstI cutsite. | ||
− | |||
− | |||
===Source=== | ===Source=== | ||
Line 18: | Line 15: | ||
===References=== | ===References=== | ||
+ | |||
+ | EcnB: NCBI source number WP_012417955 | ||
+ | |||
+ | MBP-TEV: BBa_K407015 |
Latest revision as of 07:08, 13 September 2015
MBP with EcnB from Bordetella avium
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1263
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1263
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1263
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1263
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 138
Design Notes
Use in conjunction with strain-expressing T7 polymerase.
Has internal PstI cutsite.
Source
Synthesized by DNA 2.0
References
EcnB: NCBI source number WP_012417955
MBP-TEV: BBa_K407015