Difference between revisions of "Part:BBa K769201:Experience"
Ritianjiang (Talk | contribs) (→2015 LZU-China) |
Ritianjiang (Talk | contribs) (→Usage in MFC) |
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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=2015 LZU-China= | =2015 LZU-China= | ||
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In iGEM 2015, LZU-China used the part as a new reporting gene to monitor the content of pollution. We submitted a twins part [https://parts.igem.org/Part:BBa_K1755001 BBa_K1755001]. | In iGEM 2015, LZU-China used the part as a new reporting gene to monitor the content of pollution. We submitted a twins part [https://parts.igem.org/Part:BBa_K1755001 BBa_K1755001]. | ||
| − | It can increase the electric output by synthesis of riboflavin. | + | '''It can increase the electric output by synthesis of riboflavin.''' |
Besides, riboflavin itself is yellow. | Besides, riboflavin itself is yellow. | ||
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After the addition of ribB, the bacteria will be yellow. | After the addition of ribB, the bacteria will be yellow. | ||
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| + | '''We believe that we have construct a new way of measurement by MFC and the part is the crucial device for the measurement.''' | ||
== Usage in MFC == | == Usage in MFC == | ||
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[[File:LZU_iGEM_2015_ribB_picture.JPG|400px]] | [[File:LZU_iGEM_2015_ribB_picture.JPG|400px]] | ||
| − | Figure 1:Normal E.coli(left one)and E.coli with ribB(right one) | + | Figure-1:Normal E.coli(left one)and E.coli with ribB(right one) |
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| + | [[File:K1755006 new.png]] | ||
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| + | Figure-2:The production of riboflavin by overexpression of ribB. This figure shows the data from [https://parts.igem.org/Part:BBa_K1755006 K1755006](medium promoter +medium RBS + ribB(K1755001 or K769201) + terminators). | ||
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| + | We constructed [https://parts.igem.org/Part:BBa_K1755301 BBa_K1755301] (marO + RBS+ ribB + terminators) to measure the Cu2+ ions in water. We can see the relationship between riboflavin production and Cu2+ concentration. | ||
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| + | [[File:K1755301ceshi.png]] | ||
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| + | Figure-3 The concentration of riboflavin vs. Cu2+ ions. After incubation of 37h in 37C. | ||
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| + | [[File:K1755301time.png]] | ||
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| + | Figure-4 | ||
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Latest revision as of 01:48, 13 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
2015 LZU-China
In iGEM 2015, LZU-China used the part as a new reporting gene to monitor the content of pollution. We submitted a twins part BBa_K1755001.
It can increase the electric output by synthesis of riboflavin.
Besides, riboflavin itself is yellow.
After the addition of ribB, the bacteria will be yellow.
We believe that we have construct a new way of measurement by MFC and the part is the crucial device for the measurement.
Usage in MFC
Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low.
Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.
Figure-1:Normal E.coli(left one)and E.coli with ribB(right one)
Figure-2:The production of riboflavin by overexpression of ribB. This figure shows the data from K1755006(medium promoter +medium RBS + ribB(K1755001 or K769201) + terminators).
We constructed BBa_K1755301 (marO + RBS+ ribB + terminators) to measure the Cu2+ ions in water. We can see the relationship between riboflavin production and Cu2+ concentration.
Figure-3 The concentration of riboflavin vs. Cu2+ ions. After incubation of 37h in 37C.
Figure-4
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 387
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 319 - 1000COMPATIBLE WITH RFC[1000]
User Reviews
UNIQa244f183d12cd6e8-partinfo-00000001-QINU UNIQa244f183d12cd6e8-partinfo-00000002-QINU