Difference between revisions of "Part:BBa K1595020:Design"
(→References) |
|||
(3 intermediate revisions by the same user not shown) | |||
Line 9: | Line 9: | ||
Use in conjunction with strain-expressing T7 polymerase. | Use in conjunction with strain-expressing T7 polymerase. | ||
− | + | Has internal PstI cutsite. | |
===Source=== | ===Source=== | ||
Line 16: | Line 16: | ||
===References=== | ===References=== | ||
+ | EcnB: NCBI source number AGW91308 | ||
+ | |||
+ | MBP-TEV: BBa_K407015 |
Latest revision as of 06:32, 13 September 2015
MBP with EcnB from Ralstonia pickettii.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1257
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1257
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1257
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1257
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 138
Design Notes
Use in conjunction with strain-expressing T7 polymerase.
Has internal PstI cutsite.
Source
Synthesized by DNA 2.0
References
EcnB: NCBI source number AGW91308
MBP-TEV: BBa_K407015