Difference between revisions of "Part:BBa K1686045:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | The gene of CrdA comes from ''Agrobacterium sp.'' ATCC31749 and we would like to use it in ''E.coli'', so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization. | ||
===Source=== | ===Source=== | ||
| − | + | The nucleotide sequence of CrdA comes from GenBank under the accession number AECL01000028.1 | |
| − | GenBank | + | |
===References=== | ===References=== | ||
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| + | Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41. | ||
| + | ''' | ||
Latest revision as of 14:13, 12 September 2015
crdA gene with codon optimisation for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 399
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 859
Illegal AgeI site found at 828 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene of CrdA comes from Agrobacterium sp. ATCC31749 and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.
Source
The nucleotide sequence of CrdA comes from GenBank under the accession number AECL01000028.1
References
Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41.