Difference between revisions of "Part:BBa K1631001:Experience"

Latest revision as of 03:22, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1631001

If you use this part, you can estimated the expression level of barnase and evaluate its toxic activity correspond to its expression level.

Usage and Biology

We succeeded to control growth rate of E coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.

alt text

We also evaluated the growth rate by slope of next graph.

alt text

Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)

User Reviews

UNIQ58b9c8914fc9ac7c-partinfo-00000000-QINU UNIQ58b9c8914fc9ac7c-partinfo-00000001-QINU

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 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1631001===
+If you use this part, you can estimated the expression level of barnase and evaluate its toxic activity correspond to its expression level.
+<br>
+<!-- Add more about the biology of this part here -->
+===Usage and Biology===
+We succeeded to control growth rate of <i>E coli</i>.
+<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
+We measured OD600 of 3 samples for each condition.
+[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
+</p>
+We also evaluated the growth rate by slope of next graph.
+[[File:Yamanaka 001.png|600px|thumb|center|alt text]]
+<br>Plac GFP-          0.0213(/min)
+<br>Plac GFP+          0.0205(/min)
+<br>Plac Barnase GFP-  0.0222(/min)
+<br>Plac Barnase GFP+  0.0117(/min)
 ===User Reviews===