Difference between revisions of "Part:BBa K1631001"

Latest revision as of 04:00, 19 September 2015

rbs - Barnase - rbs - GFP(+LVA) - d.term

This part is a composite part of RBS(BBa_B0034), Barnase(BBa_K1631000), RBS(BBa_B0034), GFP(+LVA)(BBa_K082003), double termnator (BBa_B0015).

Usage and Biology

We succeeded to control growth rate of E. coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.

alt text

We also evaluated the growth rate by slope of next graph.

alt text

Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1018

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1631001 short</partinfo>
@@ Line 5: / Line 4: @@
 This part is a composite part of RBS(BBa_B0034), Barnase(BBa_K1631000), RBS(BBa_B0034), GFP(+LVA)(BBa_K082003), double termnator (BBa_B0015).
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+We succeeded to control growth rate of <i>E. coli</i>.
+<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
+We measured OD600 of 3 samples for each condition.
+[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
+</p>
+We also evaluated the growth rate by slope of next graph.
+[[File:Yamanaka 001.png|600px|thumb|center|alt text]]
+<br>Plac GFP-          0.0213(/min)
+<br>Plac GFP+          0.0205(/min)
+<br>Plac Barnase GFP-  0.0222(/min)
+<br>Plac Barnase GFP+  0.0117(/min)
 <!-- -->