Difference between revisions of "Part:BBa M31510:Design"

(Changes: Where and Why)
(Changes: Where and Why)
 
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==Changes: Where and Why==
 
==Changes: Where and Why==
#bp 307-312: want to remove Gene 10 (-10) promoter binding sequence
+
#bp 307-312: want to remove Gene 5 (-10) promoter binding sequence
 
#*frame = TTG ACA : Leu Thr
 
#*frame = TTG ACA : Leu Thr
 
#**change to = CTG  ACC :  Leu Thr
 
#**change to = CTG  ACC :  Leu Thr
 +
 +
==New 0-cutter site==
 +
#Acc651 at end

Latest revision as of 08:48, 28 February 2007

Changes: Where and Why

  1. bp 307-312: want to remove Gene 5 (-10) promoter binding sequence
    • frame = TTG ACA : Leu Thr
      • change to = CTG ACC : Leu Thr

New 0-cutter site

  1. Acc651 at end