Difference between revisions of "Part:BBa M31510:Design"
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==Changes: Where and Why== | ==Changes: Where and Why== | ||
− | #bp 307-312: want to remove Gene | + | #bp 307-312: want to remove Gene 5 (-10) promoter binding sequence |
#*frame = TTG ACA : Leu Thr | #*frame = TTG ACA : Leu Thr | ||
#**change to = CTG ACC : Leu Thr | #**change to = CTG ACC : Leu Thr | ||
+ | |||
+ | ==New 0-cutter site== | ||
+ | #Acc651 at end |
Latest revision as of 08:48, 28 February 2007
Changes: Where and Why
- bp 307-312: want to remove Gene 5 (-10) promoter binding sequence
- frame = TTG ACA : Leu Thr
- change to = CTG ACC : Leu Thr
- frame = TTG ACA : Leu Thr
New 0-cutter site
- Acc651 at end