Difference between revisions of "Part:BBa K1716000:Design"

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===Design Notes===
 
===Design Notes===
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The original nucleotide sequence from E. coli () was optimized for expression in Bacillus subtilis using JCat
 
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===Source===
 
===Source===

Latest revision as of 15:36, 8 October 2023


Lambda Beta recombinase optimized for expression in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 245


Design Notes

Source

Gene product from E. coli phage lambda.

References

H. H. Wang, et al., “Programming Cells by Multiplex Genome Engineering and Accelerated Evolution,” Nature, Vol. 460, No. 7257, 2009, pp. 894-898. doi:10.1038/nature08187