Difference between revisions of "Part:BBa M31502:Design"

 
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==Changes: Where and Why==
 
==Changes: Where and Why==
−
#bp 814-819: want to remove Gene 10 (-10) promoter binding sequence
+
#bp 415-420: want to remove Gene 10 (-10) promoter binding sequence
 
#*frame = TTG ACA : Leu Thr
 
#*frame = TTG ACA : Leu Thr
−
#**change to : CTG  ACC Leu Thr
+
#**change to = CTG  ACC : Leu Thr
 +
#Cut gene 2 at HpaI site. HpaI site will be a boundary for section A of M13.1 refactored genome
 +
 
 +
==New 0-cutter site==
 +
#AatII at end

Latest revision as of 08:42, 28 February 2007

Changes: Where and Why

  1. bp 415-420: want to remove Gene 10 (-10) promoter binding sequence
    • frame = TTG ACA : Leu Thr
      • change to = CTG ACC : Leu Thr
  2. Cut gene 2 at HpaI site. HpaI site will be a boundary for section A of M13.1 refactored genome

New 0-cutter site

  1. AatII at end