Difference between revisions of "Part:BBa K1696008"

Latest revision as of 14:12, 17 September 2015

RcfB expression cassette

RcfB plays a important role in acid adaptation by binding the DNA target site, ACiD-box, in P170 which then will be activated with the participation of acid. As far as we know, P170 has a pretty limited sensitivity that is around pH 6.0~6.5. To increase the sensing range of P170, it is practical to think about boosting rcfB generation. Accordingly, we intend to construct a strong promoter and a strong RBS into the system for a higher rcfB protein expression level.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 36
Illegal BsaI.rc site found at 764

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1696008 short</partinfo>
-We use a strong promoter and a strong RBS to increase the rcfB protein expression level.
+RcfB plays a important role in acid adaptation by binding the DNA target site, ACiD-box, in P170 which then will be activated with the participation of acid. As far as we know, P170 has a pretty limited sensitivity that is around pH 6.0~6.5. To increase the sensing range of P170, it is practical to think about boosting rcfB generation. Accordingly, we intend to construct a strong promoter and a strong RBS into the system for a higher rcfB protein expression level.
 <!-- Add more about the biology of this part here