Difference between revisions of "Part:BBa K1696001"

Latest revision as of 13:48, 17 September 2015

L-Lactate producing module from Lactobacillus casei

In homofermentative lactic acid bacteria, lactic acid is one of the major end products of carbohydrate fermentation. The glucose is converted to pyruvate via the Embden-Meyerhof-Parnas pathway, and in the ﬁnal step pyruvate is reduced to two isomeric forms of lactic acid by two distinct NAD-dependent, stereospeciﬁc lactate dehydrogenases: L-lactate dehydrogenase (L- LDH) (EC 1.1.1.28) and D-lactate dehydrogenase (D-LDH) (EC 1.1.1.28)[1].

Particularly, L-Lactic acid is one of the most important chiral molecules and organic acids, which can be produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase (ldhA)[2]. Similar metabolic pathway has been found in a variety of organisms, to distinguish the differences, we renamed the heterogenous L-lactate dehydrogenase ldhE while homogenous one keeps original.

Reference:

[1] Palva A, Savijoki K. Molecular Genetic Characterization of the L-Lactate Dehydrogenase Gene (ldhL)of Lactobacillus helveticus and Biochemical Characterization of the Enzyme [J]. Applied & Environmental Microbiology, 1997, 63:págs. 2850-2856. [2] Niu D, Tian K, Prior BA, Wang M, Wang Z, Lu F, Singh S (2014) Highly efficient l-lactate production using engineered Escherichia coli with dissimilar temperature optima for l-lactate formation and cell growth. Microb Cell Fact 13:78

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1696001 short</partinfo>
-The ldh gene can produce L-lactate from pyruvate, which not only increase the overall amounts of lactate but reduce the byproduct generation.
+In homofermentative lactic acid bacteria, lactic acid is one of the major end products of carbohydrate fermentation. The glucose is converted to pyruvate via the Embden-Meyerhof-Parnas pathway, and in the ﬁnal step pyruvate is reduced to two isomeric forms of lactic acid by two distinct NAD-dependent, stereospeciﬁc lactate dehydrogenases: L-lactate dehydrogenase (L- LDH) (EC 1.1.1.28) and D-lactate dehydrogenase (D-LDH) (EC 1.1.1.28)[1].
+Particularly, L-Lactic acid is one of the most important chiral molecules and organic acids, which can be produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase (ldhA)[2]. Similar metabolic pathway has been found in a variety of organisms, to distinguish the differences, we renamed the heterogenous L-lactate dehydrogenase ldhE while homogenous one keeps original.
+== Reference: ==
+[1] Palva A, Savijoki K. Molecular Genetic Characterization of the L-Lactate Dehydrogenase Gene (ldhL)of Lactobacillus helveticus and Biochemical Characterization of the Enzyme [J]. Applied & Environmental Microbiology, 1997, 63:págs. 2850-2856.
+[2] Niu D, Tian K, Prior BA, Wang M, Wang Z, Lu F, Singh S (2014) Highly efficient l-lactate production using engineered Escherichia coli with dissimilar temperature optima for l-lactate formation and cell growth. Microb Cell Fact 13:78
 <!-- Add more about the biology of this part here