Difference between revisions of "Part:BBa K1602009"
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+ | <h1><small>D</small>-Xylose dehydrogenase - xylB</h1> | ||
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+ | <i>xylB</i> encodes for a NAD<sup>+</sup> <small>D</small>-xylose dehydrogenase from <i>Caulobacter crescentus</i>. It | ||
+ | catalyzes the reaction of <small>D</small>-xylose to <small>D</small>-xylonolactone. The sequence | ||
+ | is codon optimized for the usage in <i>E. coli</i>. | ||
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+ | <!-- <img style="width: 231px; height: 900px; margin-left: 15px; margin-right: 15px;" alt="" src=" https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png"> --> | ||
+ | <img class="shrinkToFit transparent" alt="https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png" src="https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png" height="384" width="231"> | ||
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− | + | ===<h2>Characteristics</h2>=== | |
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+ | Molecular Weight | ||
+ | </td> | ||
+ | <td> | ||
+ | 26641.48 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | Residues | ||
+ | </td> | ||
+ | <td> | ||
+ | 248 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | Charge | ||
+ | </td> | ||
+ | <td> | ||
+ | -4.0 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | Isoelectric Point | ||
+ | </td> | ||
+ | <td> | ||
+ | 5.1521 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | A280 Molar Extinction Coefficients | ||
+ | </td> | ||
+ | <td> | ||
+ | 36440 (reduced) 36690 (cystine bridges) | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | [Data taken from PEPSTATS] | ||
+ | </td> | ||
+ | </tr> | ||
+ | </colgroup> | ||
+ | </table> | ||
+ | <br> | ||
+ | </body> | ||
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− | + | ===<h2>Sequence and Features</h2>=== | |
− | === | + | |
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<partinfo>BBa_K1602009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1602009 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 21:29, 18 September 2015
D-Xylose dehydrogenase - xylB
xylB encodes for a NAD+ D-xylose dehydrogenase from Caulobacter crescentus. It catalyzes the reaction of D-xylose to D-xylonolactone. The sequence is codon optimized for the usage in E. coli. | |
Characteristics
Molecular Weight | 26641.48 |
Residues | 248 |
Charge | -4.0 |
Isoelectric Point | 5.1521 |
A280 Molar Extinction Coefficients | 36440 (reduced) 36690 (cystine bridges) |
[Data taken from PEPSTATS] |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]