Difference between revisions of "Part:BBa K1675007"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1675007 short</partinfo> | <partinfo>BBa_K1675007 short</partinfo> | ||
Uses a constitutive promoter(BBa_J23119), strong RBS(BBa_B0034),glsA coding region(BBa_K1675002).Will generate glutaminase constitutively. | Uses a constitutive promoter(BBa_J23119), strong RBS(BBa_B0034),glsA coding region(BBa_K1675002).Will generate glutaminase constitutively. | ||
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| + | After the construction of acid-resistance circuit, we measured the OD600 under different pH to test the efficacy of acid resistance. The growth curve is shown in Fig.1. | ||
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| + | [[File:BIT_China_Resistance_System_pic11.jpg]] | ||
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| + | Fig.1 The growth curve of experimental group(JBG) and control | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:23, 20 September 2015
a device to express the glutaminase1 constitutively
Uses a constitutive promoter(BBa_J23119), strong RBS(BBa_B0034),glsA coding region(BBa_K1675002).Will generate glutaminase constitutively.
After the construction of acid-resistance circuit, we measured the OD600 under different pH to test the efficacy of acid resistance. The growth curve is shown in Fig.1.
Fig.1 The growth curve of experimental group(JBG) and control
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]