Difference between revisions of "Part:BBa K1641015"

 
 
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<partinfo>BBa_K1641015 short</partinfo>
 
<partinfo>BBa_K1641015 short</partinfo>
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This part can be used to construct the reporter for real-time invertase dynamics analysis.
 
This part can be used to construct the reporter for real-time invertase dynamics analysis.
  
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Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without start code and restriction enzyme activity. This sequence is absolutely compatible with brick construction method and usually less likely to make trouble.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:06, 10 September 2015

Up-side-down RBS::mcherry-ssra with FRT recognition sites

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two FRT sites at the upstream and downstream of the gene. FRT can be recognized and the middle sequence can be restored by invertase Flp. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]