Difference between revisions of "Part:BBa K1640008:Design"
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===Design Notes=== | ===Design Notes=== | ||
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− | + | Genes required for photosystem II were selected using <html><a href=http://www.biocyc.org/CHLAMY/NEW-IMAGE?type=ENZYME&object=CPLX4LZ-235>biocyc entry on <i>Chlamydomonas reinhardtii</i> photosystem II complex</a>. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using <a href=http://www.cbs.dtu.dk/services/ChloroP>ChloroP</a>, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using <a href=http://genomes.urv.es/OPTIMIZER/>Optimizer</a></html>, avoiding EcoRI, XbaI, SpeI and XbaI sites. | |
− | + | Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon. |
Latest revision as of 07:27, 18 September 2015
psbMZH
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Genes required for photosystem II were selected using biocyc entry on Chlamydomonas reinhardtii photosystem II complex. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using ChloroP, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using Optimizer, avoiding EcoRI, XbaI, SpeI and XbaI sites.
Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon.