Difference between revisions of "Part:BBa K1859022:Experience"

Latest revision as of 05:12, 25 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859022

[iGEM gifu 2015] .

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane G shows this sample.

left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.

User Reviews

UNIQb6b1a8c283fd4fb6-partinfo-00000001-QINU UNIQb6b1a8c283fd4fb6-partinfo-00000002-QINU

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 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1859022===
+<html>
+<p>
+<a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015]
+ </a> .
+</p>
+<p>
+We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
+We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
+Lane G shows this sample.</p><br><br>
+<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img>
+<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img>
+<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img>
+<br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
+<br><br>
+<p>
+There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.</p><br>
+</html>
 ===User Reviews===