Difference between revisions of "Part:BBa K1642002"
 
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<partinfo>BBa_K1642002 short</partinfo>
 
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[[File:SJTUB PcpcG2.png|200px|thumb|left|alt text]]
 
  
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CcaS phosphorylates CcaR under green light and phosphorylated CcaR binds to promoter of cpcG2 to induce expression of cpcG2.
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[[Image:SJTUB_PcpcG2.png|400px|left|Figure1. Green light sensing expression system.]]
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PcpcG2 is a green-light inducible promoter from ''synechocystis sp.'' PCC6803 and and control mechanism is shown in Figure 1. CcaS phosphorylates CcaR under green light and phosphorylated CcaR binds to promoter of ''cpcG2'' to induce expression of ''cpcG2''(Abe, K. et al. 2014).
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There is an EcoRI recognition site(GAATTC)in the orginal sequence of PcpcG2 from 14bp to 20bp. In our plasmid for transformation of ''Synechocystis sp.''PCC 6803, we maintained the orginal sequence. After we cloned our composite part BBa_K1642010 which contained PcpcG2, we did site-directed mutagenesis by PCR. And the present sequence from 14bp to 20bp is GAATAC.
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1642002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1642005 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1642002 parameters</partinfo>
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<partinfo>BBa_K1642005 parameters</partinfo>
 
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Latest revision as of 01:27, 19 September 2015

PcpcG2

Figure1. Green light sensing expression system.

PcpcG2 is a green-light inducible promoter from synechocystis sp. PCC6803 and and control mechanism is shown in Figure 1. CcaS phosphorylates CcaR under green light and phosphorylated CcaR binds to promoter of cpcG2 to induce expression of cpcG2(Abe, K. et al. 2014).

There is an EcoRI recognition site(GAATTC)in the orginal sequence of PcpcG2 from 14bp to 20bp. In our plasmid for transformation of Synechocystis sp.PCC 6803, we maintained the orginal sequence. After we cloned our composite part BBa_K1642010 which contained PcpcG2, we did site-directed mutagenesis by PCR. And the present sequence from 14bp to 20bp is GAATAC.









Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
  • 1000
    COMPATIBLE WITH RFC[1000]