Difference between revisions of "Part:BBa K1642004:Design"
 
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===Source===
 
===Source===
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Ribosome binding sites(RBS) control protein expression level to a great extent. The PcpcG2 promoter does not have a typical Shine-Dalgarno (SD) sequence upstream of the start codon. Reported weak expression may be due to the lack of a SD-like sequence[1], so we add an SD sequence on the gene under PcpcG2, hoping that the gene will express on a relatively high level.
 
  
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Highly expressed photosynthesis-related proteins in cyanobacteria, the cpcB encoded c-phycocyanin, has in their gene’s 5′-UTR the SD-like sequences AGGAG respectively. The SD-like sequence from cpcB is completely complementary to the 3′ region of 16S rRNA of Synechocystis sp. PCC6803 and is highly conserved among various cyanobacteria.
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Synthesized and added to one end of primer.
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Reported about 15-fold higher GFPuv-derived fluorescence intensity resulted from the addition of the SD-like sequence to PcpcG2 proved that the SD-like sequence function strongly in Synechocystis sp. PCC6803.
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===References===
 
===References===
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[1]Abe, K., Miyake, K., Nakamura, M., Kojima, K., Ferri, S., Ikebukuro, K., & Sode, K. (2014). Engineering of a green‐light inducible gene expression system in Synechocystis sp. PCC6803. Microbial biotechnology, 7(2), 177-183.
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''Abe, K., Miyake, K., Nakamura, M., Kojima, K., Ferri, S., Ikebukuro, K., & Sode, K. (2014). Engineering of a green‐light inducible gene expression system in Synechocystis sp. PCC6803. Microbial biotechnology, 7(2), 177-183.''

Latest revision as of 10:33, 18 September 2015

SD sequence of cpcB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

Synthesized and added to one end of primer.

References

Abe, K., Miyake, K., Nakamura, M., Kojima, K., Ferri, S., Ikebukuro, K., & Sode, K. (2014). Engineering of a green‐light inducible gene expression system in Synechocystis sp. PCC6803. Microbial biotechnology, 7(2), 177-183.