Difference between revisions of "Part:BBa K1758350:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1758350 short</partinfo> | <partinfo>BBa_K1758350 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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+ | We used the codon optimized, synthesized Sequence of rcnR from <i> E.coli K12<i> under the control of a constitutive Primer(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K608002" target="_blank"> BBa_K608002</a>). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the constitutive Promoter and rcnR with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized constitutive promoter+rcnR and the pSB1C3: | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank">cm_fwd</a></p><p>CGGCATCAGCACCTTGTC</p> | ||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_rcnR_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_rcnR_rev" target="_blank">pSB1C3_rcnR_rev</a></p> | ||
+ | <p>GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT</p> | ||
+ | |||
+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank">cm_rev</a></p> <p>TATACGCAAGGCGACAAG</p> | ||
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+ | <p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_kPrm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_kPrm_fwd" target="_blank"> pSB1C3_kPrm_fwd</a></p><p>CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA </p> | ||
+ | |||
+ | </html> | ||
===Source=== | ===Source=== | ||
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+ | Synthesized, codon optimized gene of <i>E. coli</i> Synthesized by IDT | ||
+ | </html> | ||
+ | |||
===References=== | ===References=== | ||
+ | |||
+ | <html> | ||
+ | Synthesized by IDT | ||
+ | </html> |
Latest revision as of 22:48, 18 September 2015
Nickel repressor under control of constitutive promoter and strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used the codon optimized, synthesized Sequence of rcnR from E.coli K12 under the control of a constitutive Primer( BBa_K608002). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the constitutive Promoter and rcnR with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized constitutive promoter+rcnR and the pSB1C3:
CGGCATCAGCACCTTGTC
GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT
TATACGCAAGGCGACAAG
CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
Source
Synthesized, codon optimized gene of E. coli Synthesized by IDT
References
Synthesized by IDT