Difference between revisions of "Part:BBa S03642:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed RBS-Tet coding region followed by a reversed pBad promoter. | + | One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed RBS-Tet coding region followed by a reversed pBad promoter. This part is maintained in the insulated vector <partinfo>pSB1A7</partinfo> so that tetracylcine resistance expression is driven solely by the pBad promoter. This part conveys tetracylcine resistance at 50 ug/mL. |
===Source=== | ===Source=== | ||
| − | + | <partinfo>BBa_S03641</partinfo> (HixC-Tet<sub>rev</sub>-HixC) was cloned into a <partinfo>pSB1A2</partinfo> vector carrying | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 03:09, 31 January 2007
(-2,-1) HixC-TetB-RBSrev-HixC : pBadrev-HixC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1077
Illegal NheI site found at 1291 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 931
Illegal BamHI site found at 1351 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 377
Illegal NgoMIV site found at 537
Illegal NgoMIV site found at 905 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed RBS-Tet coding region followed by a reversed pBad promoter. This part is maintained in the insulated vector pSB1A7 so that tetracylcine resistance expression is driven solely by the pBad promoter. This part conveys tetracylcine resistance at 50 ug/mL.
Source
BBa_S03641 (HixC-Tetrev-HixC) was cloned into a pSB1A2 vector carrying