Difference between revisions of "Part:BBa K1709001"
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Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. | Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. | ||
The LuxI gene is fused to a His-tag facilitating gene expression measurements. | The LuxI gene is fused to a His-tag facilitating gene expression measurements. | ||
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| + | This part was verified by PCR restriction digestion and the DNA sequencing. All tests proved the correct and present BioBrick.<br/><br/> | ||
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| + | [[File:LuxI-His seq.JPG|500px|center|]] | ||
| + | Part of the sequence alignment with on top the reference sequence and on the bottom the sequencing results.<br/><br/> | ||
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| + | [[File:LuxI-His.JPG|500px|center|]] | ||
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| + | <p>Colony PCR to check the directionality of the BioBrick. The BioBrick direction is in reverse. That has no effect on its function.</p> | ||
| + | <br/> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 16:22, 26 September 2015
LuxI-His
Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. The LuxI gene is fused to a His-tag facilitating gene expression measurements.
This part was verified by PCR restriction digestion and the DNA sequencing. All tests proved the correct and present BioBrick.
Part of the sequence alignment with on top the reference sequence and on the bottom the sequencing results.
Colony PCR to check the directionality of the BioBrick. The BioBrick direction is in reverse. That has no effect on its function.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]