Difference between revisions of "Part:BBa K1583061"

 
 
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<partinfo>BBa_K1583061 short</partinfo>
 
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<p>This RBS was used in our constructs Mfp5_CsgA (<a href="https://parts.igem.org/Part:BBa_K1583104"link+_blank">BBa_K1583104</a>), CsgA_Mfp3 (<a href="https://parts.igem.org/Part:BBa_K1583105"link+_blank">BBa_K1583105</a>) and BFP_SpyCatcher_His (<a href="https://parts.igem.org/Part:BBa_K1583113"link+_blank">BBa_K1583113</a>).</p>
  
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This RBS was used in our constructs Mfp5+CsgA and CsgA+Mfp3.
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<p>The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".</p>
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CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces.
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This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
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1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K1583061 SequenceAndFeatures</partinfo>
 
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<partinfo>BBa_K1583061 parameters</partinfo>
 
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<h3>References</h3>
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<p>C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.</p>

Latest revision as of 01:52, 19 September 2015

RBS

This RBS was used in our constructs Mfp5_CsgA (BBa_K1583104), CsgA_Mfp3 (BBa_K1583105) and BFP_SpyCatcher_His (BBa_K1583113).

The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.