Difference between revisions of "Part:BBa K1679007:Design"

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__NOTOC__
 
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<partinfo>BBa_K1679007 short</partinfo>
 
<partinfo>BBa_K1679007 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We use a special RBS to make it temperature-sensitive
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We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.
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===Source===
 
===Source===
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===References===
 
===References===
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Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.

Latest revision as of 21:04, 17 September 2015

promoter+RNA thermometer+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 700
    Illegal AgeI site found at 812
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.

Source

BBa_J23101

BBa_K115001

BBa_E1010

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.