Difference between revisions of "Part:BBa K1766003"

Latest revision as of 09:41, 18 September 2015

OmpR controlled MicF

MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].

This part is intended to be used in an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766007 was also aimed to be created together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.

OmpR controlled micF

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1766003 short</partinfo>
-long description
+MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].
+This part is intended to be used in an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766007 was also aimed to be created together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.
+[[File:MicFOmpR.png|200px|thumb|left|OmpR controlled micF]]
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 33: @@
 <partinfo>BBa_K1766003 parameters</partinfo>
 <!-- -->
+'''References'''
+[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12