Difference between revisions of "Part:BBa K1666004:Design"

Latest revision as of 14:49, 6 September 2015

LsrK of LuxS/AI-2 signaling pathway in Salmonalla

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 61
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The lsrK gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).

Source

We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene lsrK(Gene ID: 1255599).

References

[1]Michiko E. Taga, Julia L. Semmelhack and Bonnie L. Bassler. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium. Mol Microbiol, 2001, 42(3): 777–793

[2]Vendeville A, Winzer K, Heurlier K, et al. Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol, 2005, 3(5): 383-396

[3]Taga ME, Miller ST, Bassler BL. Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium. Mol Microbiol, 2003, 50(4): 1411-1427.

@@ Line 11: / Line 11: @@
 ===Source===
-We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrK(Gene ID: 1255599).
+We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene ''lsrK''(Gene ID: 1255599).
 ===References===
+[1]Michiko E. Taga, Julia L. Semmelhack and Bonnie L. Bassler. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in ''Salmonella typhimurium''. Mol Microbiol, 2001, 42(3): 777–793
+[2]Vendeville A, Winzer K, Heurlier K, et al. Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol, 2005, 3(5): 383-396
+[3]Taga ME, Miller ST, Bassler BL. Lsr-mediated transport and processing of AI-2 in ''Salmonella typhimurium''. Mol Microbiol, 2003, 50(4): 1411-1427.