Difference between revisions of "Part:BBa K1666005:Design"

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===Source===
 
===Source===
  
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We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrR(Gene ID: 1255600).
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We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene ''lsrR''(Gene ID: 1255600).
  
 
===References===
 
===References===
 +
[1]Michiko E. Taga, Julia L. Semmelhack and Bonnie L. Bassler. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in ''Salmonella typhimurium''. Mol Microbiol, 2001, 42(3): 777–793
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[2]Vendeville A, Winzer K, Heurlier K, et al. Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol, 2005, 3(5): 383-396
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[3]Taga ME, Miller ST, Bassler BL. Lsr-mediated transport and processing of AI-2 in ''Salmonella typhimurium''. Mol Microbiol, 2003, 50(4): 1411-1427.

Latest revision as of 14:49, 6 September 2015


LsrR of LuxS/AI-2 signaling pathway in Salmonalla


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 267
    Illegal NgoMIV site found at 844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 43


Design Notes

The lsrR gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).

Source

We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene lsrR(Gene ID: 1255600).

References

[1]Michiko E. Taga, Julia L. Semmelhack and Bonnie L. Bassler. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium. Mol Microbiol, 2001, 42(3): 777–793

[2]Vendeville A, Winzer K, Heurlier K, et al. Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol, 2005, 3(5): 383-396

[3]Taga ME, Miller ST, Bassler BL. Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium. Mol Microbiol, 2003, 50(4): 1411-1427.