Difference between revisions of "Part:BBa K1824004"

Latest revision as of 17:47, 5 September 2015

pBAD + RNA Thermometer FourU

This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer FourU BBa_K1824560 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Figure 1: Possible secondary structure of RNAT FourU.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1824004 short</partinfo>
-This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer FourU K1824560 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
+This is a composite regulatory part that consisting of pBAD <partinfo>BBa_I13453</partinfo> promoter and RNA Thermometer FourU <partinfo>BBa_K1824560</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
+[[Image:XJTLU-FourU.jpg|450px]]
+<br>'''Figure 1:''' Possible secondary structure of RNAT FourU.
+===Characterization===
 <!-- Add more about the biology of this part here