Difference between revisions of "Part:BBa K1824001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1824001 short</partinfo> | <partinfo>BBa_K1824001 short</partinfo> | ||
− | This is a composite regulatory part that consisting of constitutive promoter | + | This is a composite regulatory part that consisting of constitutive promoter <partinfo>J23119</partinfo> and RNA Thermometer FourU <partinfo>BBa_K1824559</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted. |
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+ | [[Image:XJTLU-FourU.jpg|450px]] | ||
+ | <br>'''Figure 1:''' Possible secondary structure of RNAT FourU. | ||
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+ | ===Characterization=== | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 15:54, 5 September 2015
Constitutive Promoter J23119 + RNA Thermometer FourU
This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer FourU BBa_K1824559 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
Figure 1: Possible secondary structure of RNAT FourU.
Characterization
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]