Difference between revisions of "Part:BBa K1613017"
 
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This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP.
 
This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP.
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The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.
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The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 with a constitutive promoter J23102 and RFP to make them function.
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Whether lead can enter e.coli or not:
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By using gold nanoparticles(AU-NPs), we measured the lead absorption of e.coli. The results showed that the longer e.coli put in Pb2+ or the higher Pb2+ concentration, the more lead entered the e.coli.
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[[File:Pb absorption-1.jpg]]
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[[File:Pb absorption-2.jpg]]
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'''USE AND BIOLOGY'''<br>
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iGEM16_HSiTAIWAN: Measure fluorescence of lead detector
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https://static.igem.org/mediawiki/2016/d/d5/HSiTAIWAN_DESCRIPTION_upload_K1613017.jpeg
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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'''USE AND BIOLOGY'''<br>
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[[file: HSiTAIWAN_BACKGROUND_PROJECT_Pb_Figure_7_Fluorescence_of_Lead_Biosensor.jpeg]]
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  <p class="contentP">Figure 9 Fluorescence of Lead Biosensor (Part BBa_K1961006) </p>
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<img src="https://static.igem.org/mediawiki/2016/4/4f/HSiTAIWAN_BACKGROUND_PROJECT_Pb_Figure_8_Fluorescence_OD_of_Lead_Biosensor.jpeg"style="width:600px;height:400px;">
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    <p class="contentP">Figure 10 Fluorescence/OD of Lead Biosensor (Part BBa_K1961006) </p>
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Latest revision as of 10:14, 13 October 2016

K1613017 is a new part which can detect lead (Pb) ions in the liquid.

This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 with a constitutive promoter J23102 and RFP to make them function.


Whether lead can enter e.coli or not: By using gold nanoparticles(AU-NPs), we measured the lead absorption of e.coli. The results showed that the longer e.coli put in Pb2+ or the higher Pb2+ concentration, the more lead entered the e.coli.

Pb absorption-1.jpg

Pb absorption-2.jpg


USE AND BIOLOGY
iGEM16_HSiTAIWAN: Measure fluorescence of lead detector

HSiTAIWAN_DESCRIPTION_upload_K1613017.jpeg





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 686