Difference between revisions of "Part:BBa K1720002:Design"
(→Design Notes:) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
| − | |||
| − | + | ||
| + | ===Design Notes:=== | ||
| + | We cloned this basic part into a psb1c3 vector.We designed a hypoxia responsive CMV promotor by inserting a hypoxia responsive element （HRE） to CMV promotor.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism. | ||
| + | |||
| + | ===References=== | ||
1.Rational design of minimal hypoxia-inducible enhancers | 1.Rational design of minimal hypoxia-inducible enhancers | ||
Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge | Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge | ||
Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA | Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA | ||
Latest revision as of 02:33, 18 September 2015
Design Notes:
We cloned this basic part into a psb1c3 vector.We designed a hypoxia responsive CMV promotor by inserting a hypoxia responsive element （HRE） to CMV promotor.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.
References
1.Rational design of minimal hypoxia-inducible enhancers Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA