Difference between revisions of "Part:BBa K1593997"
(→SDS-PAGE) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1593997 short</partinfo> | <partinfo>BBa_K1593997 short</partinfo> | ||
This part is used to coding a chemotactci protein,cheZ,downstream of lac promotor. | This part is used to coding a chemotactci protein,cheZ,downstream of lac promotor. | ||
| + | |||
| + | |||
| + | == lac == | ||
| + | Information of lac:see [[Part:BBa_R0010 | BBa_R0010]] | ||
| + | |||
| + | |||
| + | == cheZ == | ||
| + | Information of cheZ:see [[Part:BBa_K1593998 | BBa_K1593998]] | ||
| + | |||
| + | |||
| + | |||
| + | == SDS-PAGE == | ||
| + | After induced by IPTG in 1mM about 4 h, cheZ was found in SDS-PAGE gel, compared to the wild type (Top10) and bacteria without IPTG induction. Through this result, we concluded that cheZ has been successfully expressed with the IPTG induction. | ||
| + | |||
| + | [[File:4905.jpg]] | ||
| + | |||
| + | |||
| + | |||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 16:03, 18 September 2015
R0010-RBS-cheZ
This part is used to coding a chemotactci protein,cheZ,downstream of lac promotor.
lac
Information of lac:see BBa_R0010
cheZ
Information of cheZ:see BBa_K1593998
SDS-PAGE
After induced by IPTG in 1mM about 4 h, cheZ was found in SDS-PAGE gel, compared to the wild type (Top10) and bacteria without IPTG induction. Through this result, we concluded that cheZ has been successfully expressed with the IPTG induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 601
Illegal NgoMIV site found at 736 - 1000COMPATIBLE WITH RFC[1000]