Difference between revisions of "Part:BBa K1613018:Design"

 
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===Design Notes===
 
===Design Notes===
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When connection k1555000 and RFP, there was no capable restriction enzyme to use. So we need to add capable restriction enzyme on primer to solve it.  
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We use promoter activated by cueO first. But after discussion with NJAU, we found that cueO promoter was not very specific on copper ion. So we changed to use copA promoter. Which is more specific.
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+
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===Source===
 
===Source===
  
−
using part: K1555000(promoter+rbs)
+
using part: CopA  (promoter)
 +
         
 +
          B0030  (RBS)
  
−
           E1010  (Red Flourescent Protein)
+
           E1010  (RFP)
  
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           B0015  (Terminator)
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           B0015  (Terminator)
  
 
===References===
 
===References===

Latest revision as of 17:25, 26 September 2015


Device with CopA promoter to detect copper


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 882
    Illegal AgeI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use promoter activated by cueO first. But after discussion with NJAU, we found that cueO promoter was not very specific on copper ion. So we changed to use copA promoter. Which is more specific.

Source

using part: CopA (promoter) 

          B0030   (RBS)

          E1010   (RFP)

          B0015   (Terminator)

References